Lentiviral Vector Production from a Stable Packaging Cell Line Using a Packed Bed Bioreactor

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Scalable Lentiviral Vector Production Using Stable HEK293SF Producer Cell Lines

Lentiviral vectors (LV) represent a key tool for gene and cell therapy applications. The production of these vectors in sufficient quantities for clinical applications remains a hurdle, prompting the field toward developing suspension processes that are conducive to large-scale production. This study describes a LV production strategy using a stable inducible producer cell line. The HEK293 cell...

متن کامل

Perchlorate Reduction In A Packed Bed Bioreactor Using Elemental Sulfur

This study investigated perchlorate reduction by sulfur utilizing perchlorate reducing bacteria (SUPeRB). SUPeRB cultures were enriched from a denitrifying wastewater inoculum in medium containing elemental sulfur (S), crushed oyster shell, nutrients and perchlorate. Perchlorate was reduced from 5 to < 0.5 mg/L in approximately 15 days. The enrichment culture was subsequently inoculated into a ...

متن کامل

Construction of stable packaging cell lines for clinical lentiviral vector production

Lentiviral vectors are useful experimental tools for stable gene delivery and have been used to treat human inherited genetic disorders and hematologic malignancies with promising results. Because some of the lentiviral vector components are cytotoxic, transient plasmid transfection has been used to produce the large batches needed for clinical trials. However, this method is costly, poorly rep...

متن کامل

Generation of a stable packaging cell line producing high-titer PPT-deleted integration-deficient lentiviral vectors

The risk of insertional mutagenesis inherent to all integrating exogenous expression cassettes was the impetus for the development of various integration-defective lentiviral vector (IDLV) systems. These systems were successfully employed in a plethora of preclinical applications, underscoring their clinical potential. However, current production of IDLVs by transient plasmid transfection is no...

متن کامل

Development of a Packed Bed Bioreactor for Cartilage Tissue Engineering

Efficient transport of regulatory molecules is one of the main requirements for directing the growth and differentiation of bone marrow stromal cells (BMSC). We have designed and utilized a packed bed bioreactor system for cultivation of murine BMSC immobilized in alginate microbeads produced by electrostatic droplet generation. Continuous medium perfusion at velocities that are physiological f...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Molecular Therapy - Methods & Clinical Development

سال: 2020

ISSN: 2329-0501

DOI: 10.1016/j.omtm.2020.08.010